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Response of NO, activities, and relative mRNA expressions of NR to cold stress in cucumber seedlings. (A) NO content; (B) NR activity and NR-relative mRNA expression; and (C) NO inverted fluorescence microscope imaging. Seedlings were treated at 5°C for 24 h, and sampled every 3 h. Data are represented as mean ± SD (n = 3). Different letters indicate a significant difference between samples according to Duncan's new multiple range test (p < 0.05).
