Fig. 1.

scRNA-Seq analysis of adult human cornea and conjuctiva (see also Figures S1-S12 and Table S1). A) Microphotograph of adult human ocular surface before scRNA-Seq analysis (left panel) and UMAPs of each adult human cornea and conjunctiva (middle panel). Following data integration and analysis, an integrated UMAP revealing the presence of 21 cell clusters was generated (right panel); B) Violin plots showing the presence of key markers for stem, progenitor and differentiated cells in the epithelial, stromal, and endothelial compartments.

Abbreviations for panel 1A: BV – blood vessels, CB – corneal basal epithelium, CS – corneal superficial epithelium, CSB – corneal suprabasal epithelial cells, CjB – conjunctival basal epithelium, CjS – conjunctival superficial epithelium, CE-corneal endothelium, CSK – corneal stroma keratocytes, CSSCs – corneal stromal stem cells, FCEC – fibroblastic corneal endothelial cells, IC1 - immune cells 1, IC2 – immune cells 2, LF – limbal fibroblasts, LSB – limbal suprabasal epithelial cells, LSK – limbal stroma keratocytes, LNCPs – limbal neural crest progenitors, LPCs –limbal progenitor cells, LS – limbal superficial epithelium, LV – lymphatic vessels, Mel - melanocytes, RBC – red blood cells, Additional abbreviations for panel 1B: Conj– conjunctival Ep-epithelium, Fib-fibroblasts, End-endothelial.