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. 2021 Jul;21:279–298. doi: 10.1016/j.jtos.2021.03.010

Fig. 2.

Fig. 2

Novel markers for LPCs. A) Violin plots showing the expression of five transcripts (GPHA2, CASP14, MMP1, MMP10 and AC093496.1), which are highly and predominantly expressed in LPCs (cluster 9); B-D) IF analysis showing overlapping GPHA2 and KRT15 expression (B, B′, B′), rare co-localisation of GPHA2 with Ki67 expression shown by white arrow (C) and overlapping GPHA2 and MMP10 expression in the limbal crypts (D); E-G) IF showing expression of ΔNp63 (E), p27 (F) and CEBPδ (G) in the limbal crypts; H–I) faint background like-staining of GPHA2 in the conjunctival (H) and central cornea region (I); J-L) MMP1 is expressed in the conjunctival (J), limbal epithelium (K) and corneal epithelium(L); M − P), MMP10 expression is found in the limbal (M), conjunctival (O) and corneal epithelium (P); KRT15 expression in the limbal and corneal epithelium shown in N and Q panels represent sister sections to MMP10 expression in M and P panels; R) Representative negative control immunostaining. Representative images from four different human cornea and conjunctiva samples are shown. Scale bars: 50 μm. Nuclear staining indicated by Hoechst in blue colour.