Fig. 6.

Expansion of LECs in vivo leads to loss of GPHA2 expression and acquisition of markers associated with proliferative limbal basal epithelial cells (see also Figures S17, S18 and Table S8). A) Representative photo showing a human cornea with a limbal dysplasia; B) UMAP of limbal dysplasia sample integrated with ex vivo expanded LECs (Fig. 5A) and the four adult human cornea/conjunctival samples (Fig. 1A) showing the presence of six additional clusters in the cornea with limbal dysplasia. Although all cluster annotations are the same as in Fig. 1A, during the integration process the original cluster 7 was combined with original cluster 2 in the integrated cluster 6 and clusters 18 and 11 were combined in the integrated cluster 11; C) Comparative heatmap showing the differentially expressed genes between the six additional clusters found in the cornea with limbal dysplasia; D) Comparative heatmap showing differentially expressed genes between LPCs (cluster 9) and the proliferative basal limbal epithelium I-III corresponding to additional clusters 1, 3, 5 in the cornea with limbal dysplasia.