Table 3.

Structure and ANO1 inhibitory IC₅₀ of compounds 47–53.

Compd.	R1	R2	R3	R4	IC50 (μmol/L)a
1	–	–	–	–	2.1
47	H	H	H		12.59
48	OCH3	H	H		28.94
49	H	H	H		11.29
50	H	H	H		1.91
51	H	H	H		2.21
52	H	CH3	CH3		3.33
53	–	–	–	–	5.15

IC₅₀ value was determined by YFP fluorescence quenching assay. Data are represented by mean ± SEM, n = 3.