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Free PTX level released from the nanoparticles in (A and B) 4T1 cells and (C and D) 3T3 cells for 4 or 24 h. The mean intracellular PTX release in 4T1 cells for (E) 4 or (F) 24 h, compared with the same nanoparticles incubation with 3T3 cells at the same time-point. Data are presented as mean ± SD (n = 3). n.s., not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
