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. 2021 Jul 23;9:659680. doi: 10.3389/fcell.2021.659680

FIGURE 2.

FIGURE 2

Downregulated NAP1L1 expression attenuated cell proliferation. (A) Quantitative RT-qPCR data to measure gene expression after shRNA-NAP1L1 lentivirus or NC lentivirus transfection into HCCLM3 and Huh7 cells. (B) Efficiency of silencing NAP1L1 by Western blot analysis. (C) MTT assays indicated that shRNA-NAP1L1 inhibited the proliferation Continued in vitro. (D) Downregulated NAP1L1 expression suppressed plate clone formation. (E) EdU assay showed that the downregulation of NAP1L1 suppressed the proliferation in vitro (scale bar: 200 μm). (F) Xenograft tumor of nude mice showed that the average weight and volume of tumors decreased in the shRNA-NAP1L1 group compared with the NC group. (G) Hematoxylin–eosin staining in xenograft tumor of nude mice and expression of PCNA and Ki-67 were measured via IHC staining in the xenograft tumor of nude mice (×100 visual field, scale bar: 200 μm, ×400, scale bar: 50 μm). Data are presented as mean ± SD from three independent experiments. *p < 0.05 vs. control; **p < 0.01; ***p < 0.001.