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. 2021 Feb 19;16(10):2109–2120. doi: 10.4103/1673-5374.303537

Table 1.

Real-time polymerase chain reaction primers for quantification of gene expression

Target Sequence (5’ to 3’)
Il-1β Sense: GAC CTG TTC TTT GAA GTT GAC G
Anti-sense: CTC TTG TTG ATG TGC TGC TG
Tnf-α Sense: AGA CCC TCA CAC TCA GAT CA
Anti-sense: TCT TTG AGA TCC ATG CCG TTG
Arg-1 Sense: GAA TGG AAG AGT CAG TGT GGT
Anti-sense: AGT GTT GAT GTC AGT GTG AGC
Tgf-β1 Sense: GCG GAC TAC TAT GCT AAA GAG G
Anti-sense: CCG AAT GTC TGA CGT ATT GAA GA
Nos2 Sense: GAC TGA GCT GTT AGA GAC ACT T
Anti-sense: CAC TTC TGC TCC AAA TCC AAC
Il-10 Sense: GTC ATC GAT TTC TCC CCT GTG
Anti-sense: ATG GCC TTG TAG ACA CCT TG
Il-6 Sense: AGC CAG AGT CCT TCA GAG A
Anti-sense: TCC TTA GCC ACT CCT TCT GT
Ccl-3 Sense: CCT TGC TGT TCT TCT CTG TAC C
Anti-sense: CGA TGA ATT GGC GTG GAA TC
Ccl-2 Sense: CAT CCA CGT GTT GGC TCA
Anti-sense: AAC TAC AGC TTC TTT GGG ACA
Cxcl-10 Sense: ATT TTC TGC CTC ATC CTG CT
Anti-sense: TGA TTT CAA GCT TCC CTA TGG C
Actb Sense: GGC TGT ATT CCC CTC CAT CG
Anti-sense: CCA GTT GGT AAC AAT GCC ATG T

Actb: β-Actin; Arg-1: arginase 1; Ccl: C-C motif chemokine ligand; Cxcl: CXC motif chemokine ligand; Il: interleukin; Nos2: nitric oxide synthase 2; Tgf-β1: transforming growth factor β1; Tnf-α: tumor necrosis factor α.