Figure 2.

Effect of iPSC-NPC-derived exosomes on OGD-induced neuronal viability.

(A) Immunopositive expression of β-tubulin 3 (green, stained by Alexa 488) in neurons 48 hours after oxygen-glucose deprivation (OGD) treatment. The results indicated that OGD treatment led to lower immunopositivity of β-tubulin, while iPSC-NPC-derived exosomes (OGD+iNPC-exo) led to significantly higher β-tubulin immunopositivity. Scale bar: 50 μm. (B) The cell viability of neurons was determined using the CCK-8 assay. (C) Quantitative results for brain-derived neurotrophic factor (BDNF) levels in the culture medium of cultured neurons. Data are presented as mean ± SD. *P < 0.05, ***P < 0.001, vs. control group; #P < 0.05, ##P < 0.01, vs. OGD group (one-way analysis of variance with post hoc Bonferroni test). All experiments were repeated three times.