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. 2021 Aug 6;188:391–403. doi: 10.1016/j.ijbiomac.2021.08.026

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Fig. 2 — N protein purification using Protocol 1.

A) A schematic representation of the N protein purification process according to Protocol 1. For more details, see the Materials and Methods section.

B) Preparative SEC on the Superdex 200 column. Fractions containing the N protein are marked with the grey colour.

C) BlueStain Sensitive-stained SDS-PAGE analysis of the N protein samples presenting the purification procedure. Lane 1 - T, the total bacterial protein fraction; lane 2 - S, the soluble proteins fraction; lane 3 - FT, the fraction containing proteins not bound to the Ni²⁺-NTA resin; lane 4 FTN, proteins washed from the resin after digestion with dtUD1protease; lane 5 - elu, fractions eluted from the Ni²⁺-NTA resin; lane 6 - INJ, combined fractions injected on the Superdex 200 column; lanes 7-9, the main peak from the Superdex 200 column, containing N protein.