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. 2021 Aug 6;188:391–403. doi: 10.1016/j.ijbiomac.2021.08.026

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© 2021 Published by Elsevier B.V.

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Fig. 4 — N protein purification using Protocol 2.

A) Scheme of the N protein purification process according to Protocol 2. For more details, see the Materials and Methods section.

B) Chromatography on the Heparin Sepharose columns. Fractions containing the N protein are marked with the grey colour. Fractions analysed with SDS-PAGE are marked under the graph.

C) BlueStain Sensitive-stained SDS-PAGE analysis of the N protein samples presenting the results of Heparin Sepharose column purification step. Lane 1 - INJ, combined fractions loaded on Heparin Sepharose column; lane 2 - M, molecular weight marker; lanes 3-6, elution profile. D) Preparative SEC on two connected in series Superdex 200 columns. Fractions containing the N protein are marked with the grey colour. Fractions analysed with SDS-PAGE are marked under the graph.

E) BlueStain Sensitive-stained SDS-PAGE analysis of the N protein samples presenting the SEC purification step. Lane 1 - M, molecular weight marker; lane 2 - INJ, combined fractions eluted from the Heparin-Sepharose column, loaded on the Superdex 200 columns; lanes 3-5, elution profile.