TABLE 1.
Distribution of genotype and allele frequencies along with confidence intervals among the study areas.
| SNP | Genotype/allele | Study areas | p-value a | ||||
|---|---|---|---|---|---|---|---|
| PLC (n = 18) | PVL (n = 21) | NRP (n = 57) | MCP (n = 44) | GNP (n = 80) | |||
| CYP2D6*10 rs1065852 (100 C > T) | C/C | 16 (88.9%) | 16 (76.2%) | 40 (70.2%) | 36 (81.8%) | 64 (80%) | 0.7681 |
| 95%CI: 65.2–98.6% | 95%CI: 52.8–91.8% | 95%CI: 56.6–81.6and | 95%CI: 67.3–91.8% | 95%CI: 69.6–88.1% | |||
| C/T | 2 (11.1%) | 4 (19.0%) | 15 (26.3%) | 7 (15.9%) | 15 (18.7%) | — | |
| 95%CI: 1.4–34.7% | 95%CI: 5.4–41.9% | 95%CI: 15.5–39.7% | 95%CI: 6.6–30% | 95%CI: 10.9–29% | |||
| T/T | 0 (0%) | 1 (4.8%) | 2 (3.5%) | 1 (2.3%) | 1 (1.3%) | — | |
| 95%CI: 0.0–18.5% | 95%CI: 0.1–23.8% | 95%CI: 0.4–12.1% | 95%CI: 0.06–12% | 95%CI: 0.03–6.8% | |||
| Allele C | 34 (94.4%) | 36 (85.7%) | 95 (83.3%) | 79 (89.8%) | 143 (89.4%) | 0.3417 | |
| 95%CI: 81.3–99.3% | 95%CI: 71.5–94.6% | 95%CI: 75.2–89.7% | 95%CI: 81.5–95.2% | 95%CI: 83.5–93.7% | |||
| Allele T | 34 (94.4%) | 6 (14.3%) | 19 (16.7%) | 9 (10.2%) | 17 (10.6%) | — | |
| 95%CI: 0.7–18.7% | 95%CI: 5.4–28.5% | 95%CI: 10.3–24.8% | 95%CI: 4.8–18.5% | 95%CI: 6.3–16.5% | |||
| HWE | 1 | 0.338 | 0.6383 | 0.3647 | 1 | — | |
| CYP2D6*4 rs3892097 (1846 C > T) | C/C | 8 (44.4%) | 11 (52.4%) | 28 (49.1%) | 19 (43.2%) | 38 (47.5%) | 0.5193 |
| 95%CI: 21.5–69.2% | 95%CI: 29.8–74.2% | 95%CI: 35.6–62.7% | 95%CI: 28.3–59% | 95%CI: 36.2–59% | |||
| C/T | 9 (50.0%) | 9 (42.9%) | 21 (36.8%) | 22 (50%) | 28 (35%) | — | |
| 95%CI: 26–74% | 95%CI: 21.8–65.9% | 95%CI: 24.4–50.6% | 95%CI: 34.6–65.4% | 95%CI: 24.7–46.5% | |||
| T/T | 1 (5.6%) | 1 (4.8%) | 8 (14%) | 3 (6.8%) | 14 (17.5%) | — | |
| 95%CI: 0.1–27.3% | 95%CI: 0.1–23.8% | 95%CI: 6.3–25.8 | 95%CI: 1.4–18.7% | 95%CI: 9.9–27.6% | |||
| Allele C | 25 (69%) | 31 (74%) | 77 (68%) | 60 (68%) | 104 (65%) | 0.8616 | |
| 95%CI: 51.9–83.6% | 95%CI: 58–86.1% | 95%CI: 58.1–76% | 95%CI: 57.4–77.7% | 95%CI: 57.1–72.4% | |||
| Allele T | 11 (31%) | 11 (26%) | 37 (32%) | 28 (32%) | 56 (35%) | — | |
| 95%CI: 16.3–48.1% | 95%CI: 13.9–42% | 95%CI: 24–41.9% | 95%CI: 22.3–42.6% | 95%CI: 27.6–42.9% | |||
| HWE | 1 | 1 | 0.233 | 0.488 | 0.048 | — | |
| CYP2D6*2 rs16947 (2850 G > A) | G/G | 16 (88.9%) | 15 (71.4%) | 42 (73.7%) | 37 (84.1%) | 65 (81.2%) | 0.8048 |
| 95%CI: 65.3–98.6% | 95%CI: 47.8–88.7% | 95%CI: 60.3–84.5% | 95%CI: 69.9–93.5% | 95%CI: 71–89.1% | |||
| G/A | 2 (11.1%) | 5 (23.8%) | 13 (22.8%) | 6 (13.6%) | 14 (17.5%) | — | |
| 95%CI: 1.4–34.7% | 95%CI: 8.2–47.2% | 95%CI: 12.7–35.8% | 95%CI: 5.2–24.3% | 95%CI: 9.9–27.6% | |||
| A/A | 0 | 1 (4.8%) | 2 (3.5%) | 1 (2.3%) | 1 (1.2%) | — | |
| 95%CI: 0–18.5% | 95%CI: 0.1–23.8% | 95%CI: 0.4–12.1% | 95%CI: 0.06–12% | 95%CI: 0.03–6.8% | |||
| Allele G | 34 (94.4%) | 35 (83.3%) | 97 (85.1%) | 80 (90.9%) | 144 (90%) | 0.3366 | |
| 95%CI: 81.3–99.3% | 95%CI: 68.6–93% | 95%CI: 77.2–91.1% | 95%CI: 82.9–95.6% | 95%CI: 84.3–94.2% | |||
| Allele A | 2 (5.6%) | 7 (16.7%) | 17 (14.9%) | 8 (9.1%) | 16 (10%) | — | |
| 95%CI: 0.7–18.7% | 95%CI: 7–31.4% | 95%CI: 8.9–22.8% | 95%CI: 4–17.1% | 95%CI: 5.8–15.7% | |||
| HWE | 0.448 | 0.59 | 0.294 | 0.564 | 0.448 | — | |
| CYP2D6*41 rs28371725 (2988 G > A) | G/G | 16 (100%) | 15 (100%) | 42 (100%) | 43 (97.73%) | 65 (100%) | n.d |
| 95%CI: 79.4–100% | 95%CI: 78.2–100% | 95%CI: 91.6–100% | 95%CI: 88–100% | 95%CI: 94.5–100% | |||
| G/A | 0 (0%) | 0 (0%) | 0 (%) | 1 (2.27%) | 0 (%) | — | |
| 95%CI: 0.06–12% | |||||||
| A/A | 0 (0%) | 0 (0%) | 0 (%) | 0 (0%) | 0 (0%) | — | |
| Alleles | — | — | — | — | — | — | |
| G | 32 (100%) | 30 (100%) | 84 (100%) | 87 (98.86%) | 130 (100%) | n.d | |
| 95%CI: 89.1–100% | 95%CI: 88.4–100% | 95%CI: 95.7–100% | 95%CI: 93.8–100% | 95%CI: 97.2–100% | |||
| A | 0 (0%) | 0 (0%) | 0 (%) | 1 (1.14%) | 0 (0%) | — | |
| 95%CI: 0.03–6.2% | |||||||
| HWE | n.d | n.d | n.d | 1 | n.d | — | |
Frequencies were determined by direct counting considering the total number of subjects genotyped from each region. Confidence intervals were estimated using the Clopper-Pearson exact method.
a
p values were determined for comparisons between genotype distributions of the different areas using a Chi-square test.
PLC = Plácido de Castro; PVL = Porto Velho; NRP Novo Repartimento; MCP = Macapá; GNP = Goianésia do Pará.
HWE = Hardy-Weinberg equilibrium (p-value).