Table 2.
Comprehensive list of studies with use of genetics in pseudoislets
| Delivery | Genetic modification | Species | Reference(s) | Application |
|---|---|---|---|---|
| Lentiviruses | Gain of function | Human | Zaldumbide et al. (2013) | Misexpression of immune-evasion proteins |
| Arda et al. (2016) Peiris et al. (2018) Bevacqua et al. (2021a) |
Misexpression of transcription factors Misexpression of transcription factors Misexpression of transcription factors |
|||
| Rats | Caton et al. (2003) | Misexpression of various connexins | ||
| Lentiviruses | Loss of function (shRNAs) | Human | Peiris et al. (2018) | Silencing of transcription factor |
| Harata et al. (2018) | Silencing of glucokinase | |||
| Liu et al. (2019) | Technical report | |||
| Liu et al. (2020) | Silencing of adipose triglyceride lipase | |||
| Bevacqua et al. (2021a) | Silencing of transcription factor | |||
| Lentiviruses | Knockout (indels/deletions) | Human | Bevacqua et al. (2021b) | CRISPR/Cas9 knockout of transcription factors and noncoding regulatory DNA |
| Lentiviruses | Endogenous gene activation | Human | Bevacqua et al. (2021b) | CRISPR-A of a transcription factor and enhancer |
| Adenoviruses | Gain of function | Human | Walker et al. (2020) | Misexpression of biosensor (GCAMP), study of cell-cell interactions |
| Furuyama et al. (2019) | Reprogramming of α-cells via ectopic expression of β-cell transcription factors | |||
| Mice | van Krieken et al. (2019) | Amplification of the V1b receptor signaling | ||
| Herpes simplex virus | Gain of function | Human | Rabinovitch et al. (1999) | Misexpression of antiapoptotic gene (bcl-2) |