Skip to main content
. 2021 Jul 15;22(3):1016. doi: 10.3892/etm.2021.10448

Figure 4.

Figure 4

Effects of morphine pretreatment and cPKCγ antagonist Go6983 on the number of cleaved caspase-3-positive N2a cells under OGD conditions. (A-E) Representative images of flow cytometry from N2a cells treated with OGD, Mor + OGD and Mor + Go6983 + OGD. (F) Number of cleaved caspase-3-positive OGD cells in different groups. *P<0.05 vs. sham group, #P<0.05 vs. OGD group, n=6 per group. N2a cells were fixed and stained with anti-cleaved caspase-3 antibodies and analyzed by FCM. A negative control performed with an irrelevant antibody is shown in A. The percentage of cells exhibiting active caspase-3 is indicated on each histogram as the mean ± SEM. cPKCγ, conventional γ isoform of protein kinase C; Mor, morphine; OGD, oxygen-glucose deprivation.