Table 5.
The comparison of enzymatic parameters of different immobilized laccases
| Carrier | polyacrylonitrile-biochar | pinewood nanobiochar | Fe3O4@ZIF-8 | calcium/copper alginate beads | E-CLEA | PVDF/MWCNT membrane | polyacrylamide-alginate cryogel | CTAB-KOH modified biochar | |
|---|---|---|---|---|---|---|---|---|---|
| Year | 2017 | 2018 | 2019 | 2019 | 2019 | 2021 | 2021 | 2021 | |
| Source of Laccase | Trametes versicolor | Trametes versicolor | B. amyloliquefaciens LC02 | Cyberlindnera fabianii | Trametes versicolor | Trametes hirsuta | Trametes versicolor | Trametes versicolor | |
| Enzyme loading (mg/g) | 10.1 | – | – | – | – | 30.4 | 68.7 | 57.5 | |
| Activity recovery (%) | – | – | 75.5 | 75 | – | 38.31 | – | 61.78 | |
|
Stability (RA > 50%) |
Storage | > 30 days | 25 days | > 10 days | > 21 days | > 20 days | – | – | > 20 days |
| pH | 3.0–8.0 | 3.0–5.0 | – | 3.0–9.0 | 4.0–5.0 | – | 2.5–4.0 | 2.0–6.0 | |
| Thermal | 20–60 °C | 20–60 °C | 60–80 °C | 30–70 °C | 25–55 °C | 20–70 °C | 30–70 °C | 30–66 °C | |
| Reuse | 6 cycles | 3 cycles | 5 cycles | 3 cycle | 20 cycles | 2 cycles | 7 cycles | 5 cycles | |
| Reference | [29] | [30] | [31] | [32] | [33] | [34] | [35] | This work | |