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. 2020 Aug 10;9:e1812. doi: 10.31661/gmj.v9i0.1812

Table 1. The Primer Used for Quantitative Real-Time PCR.

Objective Genes Primer Sequence
TGFβ1 Forward: 5′-TATCGACATGGAGCTGGTGA-3′
Reverse: 5′-CCTCCTTGGCGTAGTAGTCG-3′
TGFβR1 Forward: 5′-GATGGGCTCTGCTTTGTCTC-3′
Reverse: 5′-CAAGGCCAGGTGATGACTTT-3′
TWIST1 Forward: 5′-AGTCCGCAGTCTTACGAGGA-3′
Reverse: 5′-CCAGCTTGAGGGTCTGAATC-3′
E-cadherin Forward: 5′-GCGAACTGTTTGCAGAGG-3′
Reverse: 5′-CAGTGCGTGTCGTGGAGT-3′
CTNNB1 Forward: 5′-TCATGCGTTCTCCTCAGATG-3′
Reverse: 5′-AATCCACTGGTGAACCAAGC-3′
SRC Forward: 5′-GGCTACATCCCCAGCAACTA-3′
Reverse: 5′-TGAGAGGCAGTAGGCACCTT-3′
NOTCH1 Forward: 5′-TCACGCTGACGGAGTACAAG-3′
Reverse: 5′-CCACACTCGTTGACATCCTG-3′
GAPDH Forward: 5′-TGTGGGCATCAATGGATTTGG-3′
Reverse: 5′-ACACCATGTATTCCGGGTCAAT-3′