Table 1. The Primer Used for Quantitative Real-Time PCR.
Objective Genes | Primer Sequence |
TGFβ1 |
Forward: 5′-TATCGACATGGAGCTGGTGA-3′ Reverse: 5′-CCTCCTTGGCGTAGTAGTCG-3′ |
TGFβR1 |
Forward: 5′-GATGGGCTCTGCTTTGTCTC-3′ Reverse: 5′-CAAGGCCAGGTGATGACTTT-3′ |
TWIST1 |
Forward: 5′-AGTCCGCAGTCTTACGAGGA-3′ Reverse: 5′-CCAGCTTGAGGGTCTGAATC-3′ |
E-cadherin |
Forward: 5′-GCGAACTGTTTGCAGAGG-3′ Reverse: 5′-CAGTGCGTGTCGTGGAGT-3′ |
CTNNB1 |
Forward: 5′-TCATGCGTTCTCCTCAGATG-3′ Reverse: 5′-AATCCACTGGTGAACCAAGC-3′ |
SRC |
Forward: 5′-GGCTACATCCCCAGCAACTA-3′ Reverse: 5′-TGAGAGGCAGTAGGCACCTT-3′ |
NOTCH1 |
Forward: 5′-TCACGCTGACGGAGTACAAG-3′ Reverse: 5′-CCACACTCGTTGACATCCTG-3′ |
GAPDH |
Forward: 5′-TGTGGGCATCAATGGATTTGG-3′ Reverse: 5′-ACACCATGTATTCCGGGTCAAT-3′ |