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. 2021 Jul 25;11(17):8550–8569. doi: 10.7150/thno.61875

Figure 2.

Figure 2

Cellular uptake of TPCD NP and in vitro biological effects in H9C2 cells. (A) Confocal microscopy images showing time-dependent cellular uptake of Cy5/TPCD NP. (B-C) Flow cytometric curves (left) and quantitative analysis (right) of time-dependent (B) or dose-dependent (C) cellular uptake of Cy5/TPCD NP in H9C2 cells with or without DOX treatment. For time-dependent experiments, the dose of Cy5/TPCD NP was 50 µg/mL, while the incubation time was 2 h for dose-response studies. (D-F) Representative fluorescence images (D) and flow cytometric quantification (E-F) of intracellular ROS generation after stimulation with DOX and treatment with different doses of TPCD NP. (G) Intracellular MDA levels after different treatments. The protein content was measured by the BCA assay. (H-I) Expression levels of cTnI (H) and LDH (I). Scale bars: 40 μm (A, D). Data are mean ± SD (B, C, F, n = 3; G-I, n = 4). Statistical significance was assessed by the unpaired t-test for data in (B-C) and the one-way ANOVA with post-hoc LSD tests for data in (F-I). *P < 0.05, **P < 0.01, ***P < 0.001.