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. 2021 Jul 25;11(17):8430–8447. doi: 10.7150/thno.59661

Figure 1.

Figure 1

Characterization of purified adult skin and vascular progenitor populations. (A) Immune-fluorescence showing CD90 expression of culture-expanded adult skin fibroblasts (FBs, blue), intracellular keratin 14 (K14) expression of keratinocytes (KCs, green) and CD31 surface expression of endothelial colony-forming progenitor cells (ECFCs, red; all pseudo-colored). (B) Flow cytometry confirmed purity of isolated cells. Representative histograms shown; (n = 3 biological replicates; mean ± SD). (C) CFU assays showed donor-dependent clonogenicity of FBs and ECFCs (n = 3; mean ± SD). (D) KC colony on a feeder layer. (E) Vascular network formation after 12 hours on matrigel confirmed angiogenic potential of ECFCs (color-coded for automatic counting). (F) Primary skin FBs and KCs, but not ECFCs formed compact monotypic 3D spheroids (FBs = blue-, ECFCs = red-, KCs = green-labeled with nanoparticles). (A, D-F) Data from one of three biological replicates shown. Scale bar = 100 µm.