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. 2021 Jul 25;11(17):8587–8604. doi: 10.7150/thno.62572

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The cell viability of mitochondria-targeted PIH-NO in vitro. (A) Mitochondrial location of PIH-NO monitored by Mito-tracker fluorescence. Blue, green, and red represent DAPI, Mito-tracker Green, and IR780 fluorescence, respectively. The scale bar is 20 μm. (B) Cell viabilities of 4T1 cells after incubation with different groups at various doses. *p <0.05, **p <0.01 (PIH-NO with US vs. other treatments). (C) Calcein-AM (green)/PI (red) staining and (D) flow cytometry apoptosis of 4T1 cells after various treatments. The scale bar is 100 μm. Data are expressed as means ± SD (n = 3).