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. 2021 Jul 25;11(17):8587–8604. doi: 10.7150/thno.62572

Figure 9.

Figure 9

Anti-tumor immune response after PIH-NO treatment. (A) CRT and HMGB-1 immunofluorescence staining of tumor slices after various treatments. The scale bar is 100 μm. (B, C) Representative flow cytometry plots and percentages of DCs (CD11b+CD86+CD80+) in tumor-draining lymph nodes for DC maturation in vivo. (D-F) Flow cytometry analysis, percentages, and immunofluorescence staining of CD8+ T cells (gated CD3+) in tumors. The scale bar is 100 μm. (G) TNF-α expression determined by ELISA. **p <0.01 (PIH-NO with US vs. other treatments). Data are expressed as means ± SD (n = 5).