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. 2021 Jul 13;11(17):8172–8184. doi: 10.7150/thno.56737

Figure 3.

Figure 3

Cellular assays showing Cpd-1 inhibited the PPIs between AF9/ENL and DOT1L, AF4 or AFF4. (A) Co-immunoprecipitation using an antibody against the N-terminal MLL shows treatment of Molm-13 cells with Cpd-1 for 2 days dose-dependently disrupted the PPIs between MLL-AF9 and DOT1L or AF4; Upon incubation for 3 days, (B) Cpd-1 reduced the nuclear levels of DOT1L, H3K79me1 and me2, ENL and AFF4 in Molm-13 cells, while inactive Cpd-3 did not. A DOT1L inhibitor EPZ4777 only reduced those of H3K79me1 and me2, but not other proteins; (C) Cpd-1 reduced the nuclear protein levels of H3K79me1 and me2 and ENL in MV4;11 cells.