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Sequence alignment of the highly conserved fusion loop region of the E proteins for distinct flaviviruses compared to the Excivion DENV1-4 and ZIKV fusion loop-glycosylated antigens. (A) Sequence homology between ZIKV, DENV1-4, West Nile virus, Japanese encephalitis virus, and yellow fever virus as previously described (15). Sequences of DENV1-4 (B) and ZIKV (C) used for the Excivion Dengue and Zika RDTs, with position of the N-linked glycosylation sequon boxed in black.
