KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rabbit anti-Hth (1:500) | Salvany et al., 2009 | N/A |
| guinea pig anti-Hth (1:500) | Salvany et al., 2009 | N/A |
| Mouse anti-Abd-A (1:500) | Gift of Ian Duncan | Department of Biology. Washington University in St. Louis |
| Rat anti-Dsx | Sanders and Arbeitman, 2008 | RRID: AB_2569439 |
| Donkey anti-rabbit Alexa fluor 488 | Thermo Fisher Scientific | Cat# A32790; RRID: AB_2762833 |
| Goat anti-rabbit Alexa fluor 647 | Thermo Fisher Scientific | Cat# A32733; RRID: AB_2633282 |
| Donkey anti-mouse Alexa fluor 594 | Thermo Fisher Scientific | Cat# A32744; RRID: AB_2762826 |
| Donkey anti-mouse Alexa fluor 647 | Thermo Fisher Scientific | Cat# A32787; RRID: AB_2762830 |
| Goat anti-guinea pig Alexa fluor 488 | Thermo Fisher Scientific | Cat# A-11073; RRID: AB_2534117 |
| Donkey anti-rat Alexa fluor 594 | Thermo Fisher Scientific | Cat# A-21209; RRID: AB_2535795 |
| Chemicals, peptides, and recombinant proteins | ||
| Gel Loading Buffer II | Invitrogen | AM8547 |
| AccuPrime Pfx DNA Polymerase | Thermo Fisher | 12344024 |
| Trizol reagent | Life Technologies | 15596018 |
| Oligo d(T)25 Magnetic Beads | New England Biolabs | S1419S |
| Millennium RNA Markers | Thermo Fisher | AM7150 |
| Amersham Megaprime DNA Labeling System | Cytiva | RPN1606 |
| VECTASHIELD_ Vibrance Antifade Mounting Medium without DAPI | Vector Laboratories | H-1700-2 |
| VECTASHIELD_ Antifade Mounting Medium with DAPI | Vector Laboratories | H-1500-10 |
| Experimental models: Organisms/Strains | ||
| mir[C11] | Garaulet et al., 2020, Eric Lai lab. | N/A |
| mir[Δ] | Bender, 2008, Gift of Welcome Bender, Harvard University | N/A |
| Canton-S | Gift of Karla Kaun, Department of Neuroscience, Brown University. | N/A |
| VT-lines and SAG-1 split Gal4 line | Feng et al., 2014, Gift of Barry Dickson, Janelia Farm Research Campus. | N/A |
| tub-GFP-sensors for miR-iab-4 and miR-iab-8 | Tyler et al., 2008, Eric Lai lab | N/A |
| hth[BSmut] | Garaulet et al., 2020, Eric Lai lab. | N/A |
| 2xUAS-dsx-RNAi[BB] | Robinett et al., 2010 | N/A |
| UAS-hth-RNAi | Vienna Drosophila RNAi Center | 100630 |
| tubGal80ts | Bloomington Drosophila Stock Center | 7108 |
| elav-Gal4[C-155] | Bloomington Drosophila Stock Center | 458 |
| UAS-mCD8-GFP | Bloomington Drosophila Stock Center | 5137 |
| UAS-Red-Stinger | Bloomington Drosophila Stock Center | 8547 |
| UAS-dsx-RNAi[JF] | Bloomington Drosophila Stock Center | 26716 |
| dsx[1] | Bloomington Drosophila Stock Center | 1679 |
| dsx[9] | Bloomington Drosophila Stock Center | 44223 |
| UAS-dsxF | Bloomington Drosophila Stock Center | 44223 |
| UAS-hth | Gift of Richard S. Mann, Columbia University. Casares and Mann, 1998 | N/A |
| Deposited data | ||
| VNC RNA-seq data | This study. | NCBI GEO: GSE166562 |
| Software and algorithms | ||
| Prism 7 for Mac OS X | GraphPad | https://www.graphpad.com |
| Fiji ImageJ 2.0.0-rc-68/1.52e | N/A | https://imagej.nih.gov/ij |
| Rstudio Version 1.2.1335 | Rstudio | https://www.rstudio.com/ |
| IGV_2.6.3 | Broad Institute | http://software.broadinstitute.org/software/igv/ |