Figure 10.

Human tumor cells inactivated with RF+UV do not proliferate and maintain surface antigen expression. (A). Human CRL-2577 cells were inactivated with RF+UV, rested in culture overnight, and then labeled with EdU for 6 hours before being stained with DAPI and imaged. Both live and inactivated cells stained with DAPI, while only the live cells stained with EdU, indicating that RF+UV inactivation inhibited proliferation of the human tumor cells. (B). CRL-2577 and the human liver carcinoma line, Hep G2, were stained for expression of GLUT-1 and HLA prior to and following RF+UV inactivation.