Figure 4.

Immature dendritic cells (DCs) were derived from bone marrow of healthy C57Bl/6 mice and then cultured for 48 hours with RF+UV inactivated 4T1 ex vivo tumor cells. Mature DCs were measured by flow cytometry and defined as CD11c+ CD80+ CD86+ CD40+ cells and shown as percentage of CD11c+ cells (A) and by the increased MFI of Class II MHC on the CD11c+ DCs (B). (C). Spleen cells from these mice were re-stimulated in culture with inactivated 4T1 tumor cells for 72 hours and supernatants were screened for IFNγ by ELISA. (D). Serum was obtained from these mice and was tested at a 1:1000 dilution for binding to inactivated 4T1 cell line-derived tumor cells.