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RF+UV inactivated cells induce proliferation of T cells from spleens from 4T1-tumor bearing mice. Spleen cells were collected from Balb/c mice with orthotopic 4T1 tumors that were not treated. The cells were co-cultured with either live 4T1 tumor cells or RF+UV-inactivated tumor cells for 72 hours and proliferation of CD4+ and CD8+ T cells was measured using EdU.
