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. 2018 May 29;7:e1070. doi: 10.22086/gmj.v0i0.1070

Table-1. Sequences of Real-Time PCR Primers and Amplification Reactions Conditions for Evaluation of the Relative Expression of Melanocortin-4 receptor (MC4R), Kiss1, and β-actin mRNA in the Rat Arcuate Nucleus of the Hypothalamus .

Primer Sense and Anti-sense Sequence Amplicon Length (bp) Amplification Condition
MC4R GACGGAGGATGCTATGAG AGGTTCTTGTTCTTGGCTAT 116 15 min at 94℃, 40 cycles of 94℃ 10 s, 56.6℃ 15 s, and 72℃ 30 s
Kiss1 GCTGCTTCTCCTCTGTGT TAACGAGTTCCTGGGGTC 107 15 min at 94℃, 40 cycles of 94℃ 10 s, 58℃ 15 s, and 72℃ 30 s
β - actin CCACACTTTCTACAATGAGC ATACAGGGACAACACAGC 169 15 min at 94℃, 40 cycles of 94℃ 15 s, 57.8℃ 20 s, and 72℃ 30 s