Table 1.

Primers, probes and their final concentrations in Toxoplasma gondii real-time PCR assay

Assay	Names of primers and probes	Sequences of primers and probes 5′–3′	Probe labelling	Final concentration	References
Toxo529REP PCR	TalF	TGG TTG GGA AGC GAC GAG AG		800 nM	[41]
	TalR	CAT CAC CAC GAG GAA AGC GTC		800 nM	[41]
	TalP1_FAM	TGT CGT GCC AGC TGC ATT A	5′-FAM, 3′-BHQ1	200 nM	[41]
Internal control PCR, IC2 PCR	EGFP1-F	GAC CAC TAC CAG CAG AAC AC		500 nM	[42]
	EGFP2-R	GAA CTC CAG CAG GAC CAT G		500 nM	[42]
	EGFP1-Hex	AGC ACC CAG TCC GCC CTG AGC A	5′-HEX, 3′-BHQ1	160 nM	[42]