Table 2.

Overview of the individual litters of guinea pig dams inoculated with Toxoplasma gondii oocysts or serving as controls. Each line represents a single litter

Animal number	Inoculation dosea	Day of inoculationb	Duration of trialc	Litter sized	Course of pregnancy	Serology mothere	qPCR motherf	Type of offspringg	Offspring includedh	qPCR of the descendantsi
1	10	15	51	3	Birth 3 asymptomatic	−	−	N	0	− |−| −
2	10	15	27	(5)	Deceased, autolysis of fetuses	+	+	F	0	N/A
3	10	15	51	0	Resorption	+	+	N/A	N/A	N/A
4	10	30	22	1	Abortion	−	+	F	1	+
5	10	30	42	3	Birth 1 asymptomatic, 2 dead	+	+	N	1	+| +|+
6	10	30	42	4	Birth 3 asymptomatic, 1 dead	+	+	N	1	+| +| +|+
7	10	48	24	2	Birth 0 asymptomatic, 2 dead	+	+	N	1	+|+
8	10	48	19	4	Birth 0 asymptomatic, 4 dead	−	+	N	1	+| +| +|+
9	10	48	17	4	Birth 0 asymptomatic, 4 dead	−	+	N	1	+| +| +|+
10	100	15	51	0	Resorption	+	+	N/A	N/A	N/A
11	100	15	51	0	Resorption	+	+	N/A	N/A	N/A
12	100	15	51	0	Resorption	+	+	N/A	N/A	N/A
13	100	30	36	4	Birth 4 asymptomatic, 0 dead	+	+	N	1	+| +| +|+
14	100	30	36	2	Birth 0 asymptomatic, 2 dead	+	+	N	1	+|+
15	100	30	40	3	Birth 3 asymptomatic, 0 dead	+	+	N	1	+| +|+
16	100	48	11	2	Abortion	−	+	F	1	+|+
17	100	48	10	3	Abortion	−	+	F	1	+| +|+
18	100	48	20	4	Birth 4 living and malformed, 0 dead	+	+	N	1	+| +| +|+
19	500	15	51	0	Resorption	+	+	N/A	N/A	N/A
20	500	15	14	0	Deceased, resorption	+	+	N/A	N/A	N/A
21	500	15	51	(1)	Birth 0 asymptomatic, 1 dead	+	+	N	0	N/A
22	500	30	37	(1)	Abortion, autolysis of fetus	+	+	F	0	N/A
23	500	30	17	0	Euthanasia due to 20% weight loss, fetal absorption	−	+	N/A	N/A	N/A
24	500	30	13	2	Euthanasia due to CNS-symptoms	−	+	F	1	+| −j
25	500	48	9	3	Deceased	−	+	F	0	− |−| −k
26	500	48	9	4	Abortion	−	+	F	1	+| +| +|+
27	500	48	12	1	Abortion	+	+	F	1	+
28	0	0	66	1	Birth 1 asymptomatic, 0 dead	−	−	N	1	−
29	0	0	66	3	Birth 3 asymptomatic, 0 dead	−	−	N	1	−
30	0	0	66	4	Birth 4 asymptomatic, 0 dead	−	−	N	1	−

^aNumber of sporulated oocysts, ^bassumed day of pregnancy, ^cduration from inoculation to birth, abortion or euthanasia. ^dIn the bracketed litters, all fetuses were highly autolytic and were therefore excluded from further analyses. ^e“ + ” represents a positive result, “-” a negative result, ^f“ + ” represents a positive result, “-” a negative result, ^g“N”: neonatal, “F”: fetal, ^h“1”: included, “0”: not included, ⁱ“ + ” represents a positive result, “-” a negative result for each individual offspring. ^jOnly the positive embryo was included in further analyses. ^kThe liver of the mother showed a positive result. These three fetuses were not included in the further examinations