Table 3.

Individual qPCR results for each offspring separated by organ or tissue

Oocyst dose	Day of inoculation	Litter no.	Offspring	Brain	Heart	Liver	Lung	Spleen	Muscle
10	30	4	A	+	−	−	−	−	−
10	30	5	A	+	−	+	−	−	−
10	30	5	B	+	−	−	+	IH	+
10	30	5	C	+	−	−	+	−	+
10	30	6	A	+	−	−	−	−	−
10	30	6	B	+	+	IH	IH	−	−
10	30	6	C	+	+	IH	IH	−	−
10	30	6	D	+	−	−	−	−	−
10	48	7	A	+	+	+	IH	IH	+
10	48	7	B	+	+	+	−	−	+
10	48	8	A	+	+	+	IH	+	+
10	48	8	B	+	+	+	+	+	+
10	48	8	C	+	+	+	IH	+	+
10	48	8	D	+	+	+	+	+	+
10	48	9	A	+	+	+	IH	+	+
10	48	9	B	+	+	+	IH	+	+
10	48	9	C	+	+	+	IH	IH	+
10	48	9	D	+	+	+	IH	+	+
100	30	13	A	+	−	−	IH	−	−
100	30	13	B	+	−	−	IH	−	+
100	30	13	C	+	+	−	IH	−	−
100	30	13	D	+	−	+	IH	−	+
100	30	14	A	+	−	−	+	IH	+
100	30	14	B	+	+	+	IH	−	+
100	30	15	A	+	+	−	IH	−	−
100	30	15	B	+	+	−	−	−	+
100	30	15	C	+	+	−	IH	IH	+
100	48	16	A	+	−	+	IH	−	IH
100	48	16	B	+	+	+	IH	IH	IH
100	48	17	A	+	+	+	IH	-	IH
100	48	17	B	+	+	+	IH	+	IH
100	48	17	C	+	−	+	IH	+	IH
100	48	18	A	+	+	+	IH	−	+
100	48	18	B	+	+	+	IH	−	+
100	48	18	C	+	+	−	IH	−	+
100	48	18	D	+	+	+	IH	−	+
500	30	24	B	+	+	+	IH	+	−
500	48	26	A	+	+	+	IH	+	−
500	48	26	B	+	+	IH	IH	IH	−
500	48	26	C	+	+	+	IH	+	−
500	48	26	D	+	+	+	IH	IH	−
500	48	27	A	+	+	+	+	+	+
Positivity rate				100%	71%	67%	50%	38%	62%

“ + ” represents a positive result, “-” a negative result. No result could be generated for “IH" due to PCR inhibition

no. number, IH inhibition