Fig. 7.

Molecular profile of matched primary tumors and PDX models. Nucleic acids were isolated from snap frozen samples and stored at -80 °C. Purified gDNA samples were than used to detect genomic alterations using the Illumina Cancer Hotspot Panel (Illumina, Berlin, Germany) covering mutations in 50 different genes with an iSeq100 sequencing system (Illumina) according to the manufacturer’s protocols. The specific mutations along with the variant allele frequency are depicted in the boxes. Differences between primary patients’ samples and matched PDX are highlighted. Mutations were classified in the following categories: non-synonymous—variant is given in the box (in case of the same mutation in the primary and PDX: light blue, difference between primary and PDX: red/blue); synonymous: orange; benign, VUS: light-orange; neutral: grey. The latter is defined as “passenger” mutation that does not play a role in HNSCC; wt – wildtype: light grey. Created with biorender.com