Table 4.
Statistical analysis of expected probability and observed probability of tRNA gene translocation in three Polyplax species relative to their most recent common ancestor
| tRNA genes | Expected probability of translocation to be adjacent to NCR if translocation is random | Observed probability of translocation to be adjacent to NCR | Expected probability of translocation to an intergenic region if translocation is random |
Observed probability of translocation to an intergenic region |
|---|---|---|---|---|
| A | 43.75 % (21/48) | 100 % (2/2) | 56.25 % (27/48) | 0 % |
| F | 43.75 % (21/48) | 100 % (1/1) | 56.25 % (27/48) | 0 % |
| P | 45.83 % (22/48) | 100 % (1/1) | 54.12 % (26/48) | 0 % |
| T | 45.83 % (22/48) | 100 % (2/2) | 54.12 % (26/48) | 0 % |
| S1-S2 | 44.68 % (21/47) | 100 % (3/3) | 55.32 % (26/47) | 0 % |
| Average | 44.8 % | 100 % | 55.2 % | 0 % |
| Two tailed t-value | -118.61978 | 115.73698 | ||
| p-value | < 0.00001 | < 0.00001 | ||
| Significant at p < 0.01? | Yes | Yes | ||
Notes: Refer to Table 3 for tRNA gene translocation in Polyplax species. “Expected probability of translocation to be adjacent to NCR” = “total sites adjacent to NCR” / “total sites adjacent to NCR + total intergenic sites”. “Expected probability of translocation to an intergenic region” = “total intergenic sites” / “total sites adjacent to NCR + total intergenic sites”. “Observed probability of translocation to be adjacent to NCR” = “translocation adjacent to NCR” / “translocation adjacent to NCR + translocation to intergenic sites”. “Observed probability of translocation to an intergenic region” = “translocation to intergenic sites” / “translocation adjacent to NCR + translocation to intergenic sites”. T-Test was done at: https://www.socscistatistics.com/tests/studentttest/default2.aspx