Table 1.
A list of commercially available primary antibodies with tested compatibility with this protocol
| Protein | Dilution | Vendor (host species, catalog number) |
|---|---|---|
| Acetylated tubulin | 1:4000 | Sigma (mouse, T7451) |
| Polyglutamylation | 1:800 | Adipogen (rabbit, AG-25B-0030-C050) |
| γ-Tubulin | 1:500 | Sigma (mouse, T5326) |
| α-Tubulin | 1:500 | Sigma (mouse, T6074) |
| β-Tubulin | 1:250 | Abcam (rabbit, ab15568) |
| SAS-6 | 1:200 | Santa Cruz (mouse, sc-81431) |
| CP110 | 1:1000 | Proteintech (rabbit, 12780-1-AP) |
| Cep290 | 1:600 | Abcam (rabbit, ab84870) |
| Cep152 | 1:1000 | Bethyl (rabbit, A302-479A) |
| Cep63 | 1:200 | Millipore (rabbit; 06-1292) |
| Pericentrin | 1:400 | Abeam (rabbit, ab4448) |
| Centrobin | 1:200 | Abcam (mouse, ab70448) |
| CCDC41 | 1:100 | Sigma (rabbit, HPA038161) |
| SCLT1 | 1:100 | Sigma (rabbit, HPA036561) |
| FBF1 | 1:100 | Sigma (rabbit, HPA023677) |
| Cep164 | 1:500 | Proteintech (rabbit, 22227-1-AP) |
| ANKRD26 | 1:200 | GeneTex (rabbit, GTX128255) |
| ODF2 | 1:150 | Proteintech (rabbit, 12058-1-AP) |
| ARL13B | 1:150 | Abcam (rabbit, ab83879) |
| RPGRIP1L | 1:150 | Proteintech (rabbit, 55160-1-AP) |
| IFT88 | 1:150 | Proteintech (rabbit, 13967-1-AP) |
| Rootletin | 1:50 | Santa Cruz (mouse, sc-374056) |