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. Author manuscript; available in PMC: 2021 Aug 6.
Published in final edited form as: Biochemistry. 2015 Nov 25;54(48):7098–7109. doi: 10.1021/acs.biochem.5b00994

Figure 6.

Figure 6.

Interaction of O2 with wt and Y139F Cb H-NOXs. (A) Spectra of 3.6 μM anaerobic ferrous Cb H-NOX (black) and the first spectrum of rapid-scan reaction with aerobic buffers (red), [O2]final ≈ 120 μM. The y-axis represents the extinction coefficients. (B) The change of A432, the Soret of Cb H-NOX, in the reaction of 3.3 μM Cb H-NOX with various concentrations of O2 (circle) and its fit using eq 6 (solid line). (C) Spectra of 2.2 μM anaerobic Y139F Cb H-NOX before (black) and after (red) mixing with O2-saturated buffer, [O2]final ≈ 600 μM. The y-axis represents the extinction coefficients.