Figure 1. RalA is required for starvation-induced lipid droplet accumulation.

(A) Western blot confirming knockout (KO) of RalA protein expression in MEFs.

(B) Representative images of LDs in WT and RalA KO MEFs starved in HBSS media for 4 h. LDs in green (Bodipy 493/503) and nuclei in blue (DAPI). Scale bars, 20 μm.

(C) Quantification of LD area per cell from (B). At least 100 cells were analyzed per time point. n = 3. mean ± SEM. **p < 0.01.

(D) Western blot confirming knockdown of RalA protein expression in HeLa cells.

(E) Representative images of LDs in scramble control and RalA knockdown HeLa cells. Scale bars, 20 μm.

(F) Quantification of LD area per cell from (E). At least 100 cells were analyzed per time point. n = 3. mean ± SEM. *p < 0.05.

(G) Western blot confirming expression of myc-RalA in RalA KO MEFs.

(H) Representative images of LDs in RalA KO MEFs expressing myc-RalA. Scale bars, 20 μm.

(I) Quantification of LD area per cell from (H). At least 100 cells were analyzed per time point. n = 3. mean ± SEM. **p < 0.01.

(J) Representative images of LDs in MEFs stimulated with 200 μM oleic acid in DMEM for 4 h. Scale bars, 20 μm.

(K) Quantification of LD area per cell from (J). At least 100 cells were analyzed for each time point. n = 3. mean ± SEM. ***p < 0.001.

See also Figure S1.