Table 3.
Two-Way Analysis of Variance Analyses of Angiotensin-Converting Enzyme 2 and Type II Transmembrane Serine Protease Gene and Protein Expression Results
| Gene or Protein | Maternal SARS-CoV-2 Status | Fetal Sex | Interaction | Bonferroni Female Neg:Pos | Bonferroni Male Neg:Pos | Bonferroni Neg Female:Male | Bonferroni Pos Female:Male |
|---|---|---|---|---|---|---|---|
| Gene | |||||||
| ACE2 | F(1,64) = 0.003, P = .95 | F(1,64) = 3.59, P = .06 | F(1,64) = 0.627, P = .43 | P > .99 | P > .99 | P = .46 | P > .99 |
| TMPRSS2 | F(1,64) = 0.889, P = .35 | F(1,64) = 3.56, P = .06 | F (1,64) = 10.65, P = .002 | P = .63 | P = .025 | P = .006 | P > .99 |
| Protein by Western blot | |||||||
| ACE2 | F(1,52) = 0.006, P = .94 | F(1,52) = 0.0008, P = .98 | F(1,52) = 0.89, P = .35 | P = .93 | P = .89 | P = .90 | P = .92 |
| TMPRSS2 | F(1,52) = 0.02, P = .90 | F(1,52) = 0.21, P = .65 | F (1,52) = 16.57, P = .0002 | P = .027 | P = .044 | P = .014 | P = .081 |
Two-way analysis of variance followed by Bonferroni post hoc analyses was performed to determine significance. All main and interaction effects for genes and proteins of interest are represented for both fetal males and fetal females in addition to all post hoc analyses performed. Statistically significant values are bolded. “Neg” and “Pos” designate maternal SARS-CoV-2 status.
Abbreviations: ACE2, angiotensin-converting enzyme 2; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; TMPRSS2, type II transmembrane serine protease.