Table 5.

PANGO Lineage Identified by Next-Generation Sequencing Compared With Mutations Identified by Reverse-Transcription Polymerase Chain Reaction Among Severe Acute Respiratory Syndrome Coronavirus 2–Infected Healthcare Personnel

	Mutation Identified by Reverse-Transcription Polymerase Chain Reaction
	L452R	N501Y	E484K	No Mutation	Mutation Data Not Available	Total
PANGO Lineage by Next-Generation Sequencing
B.1.1.7	0	11	0	0	0	11
B.1.427	22	0	0	0	4	26
B.1.429	8	0	0	0	1	9
B.1.617	1a	0	0a	0	0	1
Not variant of concern/interest	0	0	1	57	29	87
Total	31	11	1	57	34	134

Next-generation sequencing (NGS) available for n = 134 healthcare personnel (HCP); NGS was unavailable for n = 148 HCP who were tested by Color or outside of Stanford Health Care laboratory, n = 43 with high cycle threshold values, n = 13 samples with low transcription-mediated amplification relative light unit, n = 6 samples with polymerase chain reaction amplification failure, n = 114 with insufficient quantity, n = 215 that were otherwise unavailable.

^aThis specimen had a blunted E484K amplification curve that was revealed to represent an E484Q mutation by sequencing [28].