Figure 3.

A and B, The CD8 T-cell phenotype is altered in cytomegalovirus (CMV)-infected participants. Peripheral blood mononuclear cells were isolated from CMV-negative and CMV-positive individuals. Flow cytometry was used to phenotype CD3⁺ T cells from each individual participant, specifically analyzing CD4, CD8, and CD28. The proportions of CD28 for CMV-negative (n = 22) and CMV-positive (n = 65) individuals were calculated for CD4⁺ and CD8⁺ T cells. Multivariable linear regression models were used to test for significance including age, sex, smoking status, and body mass index as covariables. A Bonferroni correction was applied and P values below .025 were considered significant. Each box represents the mean, the bars represent standard error and the dots represents each individual analyzed.