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. Author manuscript; available in PMC: 2021 Aug 6.
Published in final edited form as: Cell Rep. 2021 Jul 20;36(3):109392. doi: 10.1016/j.celrep.2021.109392

Figure 1. LYSMD3 is located in the plasma membrane of human airway epithelial cells and in sites of fungal-epithelial interactions.

Figure 1.

(A) Schematic illustration of the predicted domains of human LYSMD3.

(B) Immunoblot analysis of membrane (M) and cytosolic (C) fractions from BEAS-2B and A549 cells probed with anti-LYSMD3.

(C) Immunoblot analysis of biotinylated cell-surface proteins (eluate, 25 μL) and unbound lysate (25 μL) from BEAS-2B cells

(D)Representative images of BEAS-2B cells infected with live C. albicans SC5314 and stained for LYSMD3 (green). Scale bars, 10 μm. Accompanying histogram (right) depicts enrichment in LYSMD3 at sites of contact with the fungus, compared to sites not in contact with the fungus. Results were calculated from seven pairs of fungal contact regions and non-contact regions. IgG control staining during C. albicans infection of lung epithelial cells revealed negligible staining (data not shown). **p < 0.01 (Student’s t test). Data are from one representative experimental of three performed with similar results (mean and SD of seven samples per group).