KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-LYSMD3 | Proteintech | Cat# 24313-1-AP |
| Anti-LYSMD3, Azide Free | Proteintech | N/A |
| Anti-HSP90 | Proteintech | Cat# 13171-1-AP; RRID: AB_2120924 |
| Anti-sodium potassium ATPase (HRP) | Abcam | Cat# ab185065 |
| Anti-β-Actin | Santa Cruz Biotechnology | Cat# sc-47778; RRID: AB_626632 |
| Mouse IgG2B Isotype Control antibody | R&D | Cat# MAB004; RRID: AB_357346 |
| Anti-Human TLR2 | R&D | Cat# AF2616; RRID: AB_416645 |
| Anti-Human TLR4 | R&D | Cat# MAB1478; RRID: AB_2240713 |
| Anti-Human Dectin-1 | R&D | Cat# MAB1859; RRID: AB_2081791 |
| Anti-GFP | Abcam | Cat# ab13970; RRID: AB_300798 |
| Rabbit IgG Isotype Control antibody | Thermo Fisher Scientific | Cat# 31235; RRID: AB_243593 |
| Anti-6x-His Tag (HRP) | Thermo Fisher Scientific | Cat# MA1-21315-HRP; RRID: AB_2536989 |
| IgA2 Isotype Control | InvivoGen | Cat# maba2-ctrl; RRID: AB_11124905 |
| Anti-hTLR2-IgA | InvivoGen | Cat# maba2-htlr2; RRID: AB_11142484 |
| Mouse Anti-Rabbit IgG (HRP) | GenScript | Cat# A01827 |
| Chemicals, peptides, and recombinant proteins | ||
| Chitin oligosaccharides DP2 | Elicityl | GLU432-90%; CAS: 35061-50-8 |
| Chitin oligosaccharides DP3 | Elicityl | GLU433-90%; CAS: 41708-93-4 |
| Chitin oligosaccharides DP4 | Elicityl | GLU434-90%; CAS: 2706-65-2 |
| Chitin oligosaccharides DP5 | Elicityl | GLU435-90%; CAS: 36467-68-2 |
| Chitin oligosaccharides DP6 | Elicityl | GLU436-90%; CAS: 6734-92-5 |
| Chitin oligosaccharides DP7 | Elicityl | GLU437-90%; CAS: 38854-46-5 |
| Chitoheptaose | IsoSep | 57/11; CAS: 38854-46-5 |
| Chitin from shrimp shells | Sigma-Aldrich | C9752; CAS: 1398-61-4 |
| Chitin Magnetic Beads | NEB | E8036S |
| LPS-EB Ultrapure | InvivoGen | tlrl-3pelps |
| LPS-RS Ultrapure | InvivoGen | tlrl-prslps |
| Pam3CSK4 | InvivoGen | tlrl-pms; CAS: 112208-00-1 |
| FLA-ST Ultrapure | InvivoGen | tlrl-epstfla |
| Poly(I:C) HMW | InvivoGen | tlrl-pic; CAS: 31852-29-6 |
| Laminarin | InvivoGen | tlrl-lam; CAS: 9008-22-4 |
| WGP Soluble | InvivoGen | tlrl-wgps |
| Curdlan | InvivoGen | tlrl-curd; CAS: 54724-00-4 |
| PGN-ECndss | InvivoGen | tlrl-ksspgn |
| PGN-EK | InvivoGen | tlrl-pgnek |
| PGN-BS | InvivoGen | tlrl-pgnb3 |
| QUANTI-Blue | InvivoGen | Cat# rep-qbs |
| Extracts of Alternaria | Greer Laboratories | Cat# XPMID3A2.5; Lot# 312142 |
| Recombinant Human LYSMD3-His | Proteintech | Cat# Ag19496 |
| Recombinant Human TLR2, CF | R&D | Cat# 2616-TR |
| Recombinant Human TLR4, CF | R&D | Cat# 1478-TR |
| Recombinant Human Dectin-1, CF | R&D | Cat# 1859-DC |
| Recombinant Aequorea victoria GFP-His | Sino Biological | Cat# 13105-S07E |
| Lipofectamine RNAiMAX Transfection Reagent |
Thermo Fisher Scientific | Cat# 13778 |
| HiPerFect Transfection Reagent | QIAGEN | Cat# 301704 |
| Puromycin | InvivoGen | Cat# ant-pr |
| Critical commercial assays | ||
| ELISA MAX Standard Set Human IL-6 | BioLegend | Cat# 430501 |
| ELISA MAX Standard Set Mouse IL-6 | BioLegend | Cat# 431301 |
| Human IL-8 DuoSet ELISA | R&D | Cat# DY208 |
| ELISA MAX Standard Set Human IL-8 | BioLegend | Cat# 431501 |
| Human IL-33 Quantikine ELISA Kit | R&D | Cat# D3300B |
| ELISA MAX Standard Set Mouse MCP-1 | BioLegend | Cat# 432701 |
| ELISA MAX Deluxe Set Human CCL5 | BioLegend | Cat# 440804 |
| Mem-PER Plus Membrane Protein Extraction Kit | Thermo Fisher Scientific | Cat# 89842 |
| Cell Surface Protein Isolation Kit | BioVision | Cat# K295 |
| MTT Cell Proliferation Assay Kit | Cayman Chemical | Cat# 10009365 |
| Pierce LDH Cytotoxicity Assay Kit | Thermo Fisher Scientific | Cat# 88953 |
| Experimental models: Cell lines | ||
| BEAS-2B | ATCC | CRL-9609 |
| A549 | ATCC | CCL-185 |
| RAW 264.7 | ATCC | TIB-71 |
| NHBE | Lonza | CC-2540 |
| A549-Dual | InvivoGen | a549d-nfis |
| HEK-Blue hNOD2 | InvivoGen | hkb-hnod2 |
| hBE33 cells | Uchida et al., 2017 | N/A |
| Experimental models: Organisms/strains | ||
| C. albicans | ATCC | ATCC MYA-2876 |
| A. alternata | ATCC | ATCC 66981 |
| Oligonucleotides | ||
| Control siRNA-A | Santa Cruz Biotechnology | Cat# sc-37007 |
| LYSMD3 siRNA (h) | Santa Cruz Biotechnology | Cat# sc-91992 |
| LYSMD3 siRNA (m) | Santa Cruz Biotechnology | Cat# sc-149191 |
| ON-TARGETplus Non- targeting siRNA #1 | Dharmacon | Cat# D-001810-01 |
| ON-TARGETplus Human LYSMD3 siRNA-Individual | Dharmacon | Cat# J-024521-09 |
| Recombinant DNA | ||
| Edit-R All-in-one Lentiviral sgRNA hEF1a Non-targeting Control #1 | Dharmacon | VSGC11964 |
| Edit-R Human LYSMD3 hEF1a All-in-one Lentiviral sgRNA-no. 1 |
Dharmacon | VSGH11936-247618492 |
| hEF1a All-in-one Lentiviral sgRNA-no. 2 | Dharmacon | VSGH11936-247547638 |
| Software and algorithms | ||
| Prism Version 8 | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| VisionWorks 9 | Analytik Jena | N/A |
| ImageJ | National Institutes of Health | https://imagej.net/ |