Figure 1.

Effect of AF680 and DFO conjugation on antigen binding of the anti-IL13Rα2 Ab by flow cytometry. IL13Rα2-expressing A375 cells were incubated with 8.8-mAb (isotype control), anti-IL13Rα2-Ab, anti-IL13Rα2-Ab-AF680, or anti-IL13Rα2-Ab-DFO, followed by detection with a FITC-labeled secondary Ab. (a) AF680 conjugation to anti-IL13Rα2-Ab had no impact on A375 tumor cell binding. (b) DFO conjugation to anti-IL13Rα2-Ab had no impact on A375 tumor cell binding. Histogram labeling: Green: isotype control 8.8-mAb; Gray: anti-IL13Rα2-Ab; Red: anti-IL13Rα2-Ab-AF680 or anti-IL13Rα2-Ab-DFO