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. 2021 Jun 30;20(14):1374–1388. doi: 10.1080/15384101.2021.1944512

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Figure 2. — Silencing of HCP5 inhibits ESCC cell viability, proliferation, migration, invasion and stemness characteristics

(a) The efficiency of HCP5 knockdown in KYSE30 and EC109 cells by transfection of sh-HCP5#1/2 was accessed using qRT-PCR analysis. (b,c) Viability and proliferation of KYSE30 and EC109 cells transfected with sh-NC and sh-HCP5#1 were evaluated by MTT and EdU assays. (d) Apoptosis rate of KYSE30 and EC109 cells transfected with sh-NC and sh-HCP5#1 was measured by TUNEL assay. (e,f) Number of migrative and invasive KYSE30 and EC109 cells transfected with sh-NC and sh-HCP5#1 was assessed with Transwell assays. (g) Stemness characteristics of KYSE30 and EC109 cells after transfection of sh-NC and sh-HCP5#1 were investigated with sphere formation assay by determining number of formed spheres (per 1000 cells). (h) The expression levels of cell stemness-associated proteins (Nanog, Sox2 and Oct4) in KYSE30 and EC109 cells after transfection of sh-NC and sh-HCP5#1 were detected via western blot. **p < 0.01.