Figure 4.

UvCGBP1 regulating virulence might be independent of cutinase genes. (a) Expression of cutinase genes in the WT and ∆UvCGBP1-33 by qRT-PCR relative to β-tubulin. (b) Yeast one-hybrid analysis indicated UvCGBP1 can bind to the promoter of these four cutinase genes (Uv8b_2652, Uv8b_7184, Uv8b_7527 and Uv8b_8131). (c) Colony morphology of the WT and cutinase gene deletion mutants on PSA for 14 days. (d) Virulence assays of the WT and cutinase gene deletion mutants on rice spikelets at 21 dpi. (e) Determination of cutinase activity in 7-day-old hyphae from PSB between the ∆UvCGBP1-33, cutinase gene deletion mutants and WT. Asterisks represent significant differences between WT and mutants based on LSD at P = 0.05