Figure 5.

MiR-145-3p inhibition reverses the role of DANCR silencing in CC cells. (a) ZEB1 expression in HeLa and SiHa cells was determined using RT-qPCR and Western blot analysis; (b) Colony formation ability of HeLa and SiHa cells was determined using colony formation assay; (c) Viability of HeLa and SiHa cells was assessed using MTT assay; (d) Migration ability of HeLa and SiHa cells was assessed using scratch test; (e) Invasion ability of HeLa and SiHa cells was assessed using Transwell assay; (f) Apoptosis rate of HeLa and SiHa cells was assessed using flow cytometry; repetitions = 3; # P < 0.05 vs the sh-DANCR + inhibitor NC group; the measurement data conforming to the normal distribution were expressed as mean ± standard deviation and unpaired t-test was performed for comparisons between two groups