FIGURE 5.

Neferine reduced the production of reactive oxygen species (ROS) and prevented mitochondrial dysfunction (A) DCFH-DA staining was used to evaluate the intracellular ROS level in H9c2 cells. Fluorescence intensity was measured. Scale bar, 50 μm (B) Representative images of JC-1 staining in LPS-induced H9c2 cells. Fluorescence intensity was measured. Scale bar, 50 μm (C–E) Western blot analysis and densitometric quantification of SOD2 and iNOS protein expression in H9c2 cells (F) SOD1 and iNOS protein expression levels in septic mice were detected by Western blot (n = 6) (G, H) Densitometric quantification of SOD1 and iNOS protein expression levels (I) ATP levels in H9c2 cells were analyzed. All data are expressed as mean ± SD. All experiments were repeated at least three times. *p < 0.05, **p < 0.01, ***p < 0.001; ns: no significant difference; DCFH-DA, 2′-7′dichlorofluorescein diacetate.