FIGURE 7.

LY294002 impeded the protective effect of neferine on LPS-induced apoptosis in H9c2 cells (A–C) Western blot analysis and densitometric quantification of the protein expression levels of cleaved caspase 3 and Bcl-2 (D) Representative images of TUNEL staining in H9c2 cells (E) Quantification of TUNEL-positive cells. Scale bar, 100 μm. All data are expressed as mean ± standard deviation. All experiments were repeated at least three times. *p < 0.05, **p < 0.01, ***p < 0.001; ns: no significant difference.