FIGURE 8.

LY294002 inhibited the protective effect of neferine against LPS-induced mitochondrial dysfunction in H9c2 cells (A) DCFH-DA staining was used to evaluate the intracellular reactive oxygen species (ROS) level. Fluorescence intensity was measured. Scale bar, 50 μm (B) Representative images of JC-1 staining in H9c2 cells. Fluorescence intensity was measured. Scale bar, 50 μm (C–E) Western blot analysis and densitometric quantification of the protein expression levels of SOD2 and iNOS. All data are expressed as mean ± standard deviation. All experiments were repeated at least three times. *p < 0.05, **p < 0.01, ***p < 0.001; ns: no significant difference; DCFH-DA, 2′-7′dichlorofluorescein diacetate.