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. 2021 Jun 22;22(8):e52553. doi: 10.15252/embr.202152553

Figure 1. T affects the induction and maintenance of naïve pluripotency.

Figure 1

  1. FACS analysis of the E3: T‐GR line and scheme for the evaluation of reversion efficiency.
  2. Reversion of E3 EpiSCs from GFP‐positive (left) and GFP‐negative EpiSCs (right). Macroscopic view of AP‐stained dishes on day 5 (top). Quantification of reversion efficiency based on the number of AP‐positive colony (middle) and GFP‐positive cell (bottom).
  3. Top left: Scheme for the chimera contribution assay. Bottom left: Representative brightfield/mCherry images from chimeras at E7.5. Scale bar: 100 µm. Right: Percentage of mCherry‐positive cells in chimeric embryos measured by FACS. Blue circles and red circles represent the percentage of mCherry‐positive cells derived from control and T‐induced PSCs (GOF‐positive E3 EpiSCs [E3G], reverted E3 EpiSCs [rE3], and 2iLIF ESCs), respectively.
  4. Western blot of the indicated proteins in T‐inducible PSCs stimulated as indicated for 24 h. GAPDH was used as a loading control.

Source data are available online for this figure.