Table 2: Affymetrix chip analysis of MMP transcripts expressed in P-UAEC treated with VEGF₁₆₅, TNF-alpha, TPA, U0126, or PP2.

Pooled P-UAEC were grown to 80–90% confluence and treated with serum free medium before adding vehicle or 10 ng/ml VEGF165, 10 ng/ml TNF-alpha, 10 nM TPA, 10 uM PP2 or 10 uM U0126 for 12 hr. RNA was recovered and analyzed using Ovine ST1.0 Affymetrix chips as described in Methods. Data shown is that for MMP transcripts and is the mean fold response relative to control. Significance is as determined using TAC console. Data is from n=6 independent experimental replicates. Bold indicates values significant at P<0.05 and grey highlight indicates changes of 2-fold or more.

Cluster ID	Gene	TPA Fold	ANOVA	VEGF Fold	ANOVA	TNF Fold	ANOVA	PP2 Fold	ANOVA	U0126 Fold	ANOVA	Gene Symbol	Description
14831868	MMP1	9.26	0.000380	1.27	0.577285	3.79	0.010092	−1.32	0.410276	−2.01	0.022409	MMP1	matrix metallopeptidase 1 (interstitial collagenase)
14747122	MMP2	−1.04	0.289801	1.05	0.192794	−1.10	0.02258	−1.00	0.749364	1.04	0.103107	MMP2	matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
14831857	MMP3	1.89	0.036519	1.25	0.250130	2.74	0.001004	−7.23	0.000003	−4.87	0.000025	MMP3	matrix metallopeptidase 3 (stromelysin 1, progelatinase)
14831911	MMP7	1.11	0.489874	1.08	0.609593	1.05	0.457661	1.03	0.935785	1.10	0.779365	MMP7	matrix metallopeptidase 7 (matrilysin, uterine); matrilysin
14831879	MMP8	1.08	0.951225	−1.11	0.252445	−1.03	0.896687	−1.06	0.723733	−1.24	0.405728	MMP8	matrix metallopeptidase 8 (neutrophil collagenase)
14740499	MMP9	4.26	0.000182	1.18	0.54346	1.62	0.133407	−1.86	0.013284	−1.45	0.041844	MMP9	matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
14719461	MMP11	1.32	0.018937	1.03	0.683522	−1.01	0.820525	1.08	0.488398	1.12	0.183513	MMP11	matrix metallopeptidase 11 (stromelysin 3)
14831846	MMP12	24.72	0.000319	1.26	0.752276	7.12	0.009823	−2.23	0.042057	−2.21	0.043927	MMP12	matrix metallopeptidase 12 (macrophage elastase)
14831835	MMP13	33.89	0.000682	1.23	0.958025	4.34	0.065957	−2.00	0.119886	−2.31	0.040849	MMP13	matrix metallopeptidase 13 (collagenase 3)
14770048	MMP14	1.86	0.000432	1.05	0.836455	−1.12	0.375081	−1.04	0.534162	−1.09	0.210136	MMP14	matrix metallopeptidase 14 (membrane-inserted)
14747730	MMP15	1.06	0.235088	−1.01	0.995443	−1.02	0.831112	1.08	0.456359	−1.24	0.106583	MMP15	matrix metallopeptidase 15 (membrane-inserted)
14783462	MMP16	−1.06	0.549559	1.00	0.857197	1.01	0.310671	−1.32	0.803501	1.26	0.109459	MMP16	matrix metallopeptidase 16 (membrane-inserted)
14791388	MMP17	−1.02	0.492428	1.03	0.88133	−1.02	0.272677	−1.08	0.080985	−1.04	0.543826	MMP17	matrix metallopeptidase 17 (membrane-inserted)
14806051	MMP19	1.38	0.135573	1.04	0.802994	1.12	0.669149	−1.44	0.039653	1.13	0.838978	MMP19	matrix metallopeptidase 19
14831900	MMP20	1.09	0.261885	−1.14	0.019253	−1.02	0.904140	−1.05	0.197874	−1.05	0.751797	MMP20	matrix metallopeptidase 20
14833393	MMP24	1.12	0.061920	−1.06	0.314654	1.04	0.369383	−1.09	0.882969	−1.01	0.408488	MMP24	matrix metallopeptidase 24 (membrane-inserted)
14771712	MMP25	1.09	0.375893	1.05	0.276963	1.13	0.055192	1.02	0.583339	−1.10	0.536965	MMP25	matrix metallopeptidase 25
14831889	MMP27	1.03	0.744258	−1.24	0.005127	1.05	0.524123	−1.03	0.544841	−1.17	0.135851	MMP27	matrix metallopeptidase 27
14883630	MMP28	−1.16	0.184595	−1.03	0.957346	−1.33	0.036173	1.02	0.550302	1.31	0.004269	MMP28	matrix metallopeptidase 28
14793298	TIMP1	1.24	0.028553	1.03	0.84644	1.14	0.21381	−1.39	0.097836	−1.11	0.223265	TIMP1	TIMP metallopeptidase inhibitor 1
14748370	TIMP2	1.35	0.123444	1.05	0.800133	1.21	0.324644	−1.25	0.258587	1.35	0.120889	TIMP2	TIMP metallopeptidase inhibitor 2
14744606	TIMP3	−1.28	0.001912	−1.05	0.525343	−1.03	0.896624	−1.42	0.000707	−1.26	0.014062	TIMP3	TIMP metallopeptidase inhibitor 3
14770758	TIMP4	−1.03	0.988126	1.02	0.904207	−1.07	0.561512	1.03	0.815771	1.54	0.008201	TIMP4	TIMP metallopeptidase inhibitor 4