Figure 7.

Potential immunotoxicity of PAC and FA-ExoPAC. Female C57BL/6 mice were treated with Exo, PAC and FA-ExoPAC for four weeks. At euthanasia, spleen and bone marrow cells were collected. Splenic cells were stained with multiple fluorochrome-conjugated antibodies. Samples were run using LSRII cytometer and data was analyzed by FlowJo software. Effect of treatment was analyzed on B cells, total T-cells, T helper cells, and cytotoxic T-cells (A), macrophages, neutrophils and dendritic cells (B), B cell (C), %LSK cells (D) and T-cell proliferation (E). All statistical analysis was performed by two-way ANOVA and compared with untreated control. *, p < 0.05; **, p < 0.01; ***, p < 0.001.